Isolation of Bioactive Compounds from Fruit Body of Lentinula edodes (Berk.) Pegler and In Silico Approach using Tyrosinase Target Protein Involved in Melanin Production

Traditionally, Lentinula edodes are used in many Asian countries for the treatment of various chronic diseases. In current study, ethyl acetate fraction fruit body was obtained by standard bioassay-guided fractionation procedure. This resulted isolation three bioactive compounds (compound 1 already reported, compound 2 and 3 first time reported from this mushroom) their structures were characterized using spectroscopic techniques. Further, all studied molecular docking analysis. Compounds 1, 2, active ingredient hyperpigmentation i.e. arbutin prepared as program database files, docked with target receptor (tyrosinase, ID: 5M8L) which plays a vital role melanogenesis pathway. The study mainly focuses on better results potent tyrosinase inhibitors to down-regulate present revealed that due presence edodes, it may be preferred cosmetic product extract form obtain effective skin lightening properties or melisma. isolated edodes. further concludes could act potential lead molecule gene slight modification optimization chemical structure more effective, less toxic exhibits remarkable via inhibiting melanin production.